Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 120, Issue 3, Pages 571-587Publisher
WILEY
DOI: 10.1111/jam.13035
Keywords
anaerobic fungi; co-culture; methanogen; rumen; yak
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Funding
- Gansu Provincial Science & Technology Support Program [1104NKCA077]
- Bio-industry Base Special Program of Lanzhou Science and Technology Bureau [2015-3-140]
- National Natural Science Foundation of China [31072054]
- National Key Basic Research Program of the Ministry of Science and Technology of the People's Republic of China [2011CB100801]
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AimTo obtain co-cultures of anaerobic fungi and their indigenously associated methanogens from the rumen of yaks grazing on the Qinghai-Tibetan Plateau and investigate their morphology features and ability to degrade lignocellulose. Methods and ResultsTwenty fungus-methanogen co-cultures were obtained by Hungate roll-tube technique. The fungi were identified as Orpinomyces, Neocallimastix and Piromyces genera based on the morphological characteristics and internal transcribed spacer 1 sequences analysis. All methanogens were identified as Methanobrevibacter sp. by 16S rRNA gene sequencing. There were four types of co-cultures: Neocallimastix with Methanobrevibacter ruminantium, Orpinomyces with M.ruminantium, Orpinomyces with Methanobrevibacter millerae and Piromyces with M.ruminantium among 20 co-cultures. In vitro studies with wheat straw as substrate showed that the Neocallimastix with M.ruminantium co-cultures and Piromyces with M.ruminantium co-cultures exhibited higher xylanase, filter paper cellulase (FPase), ferulic acid esterase, acetyl esterase activities, invitro dry matter digestibility, gas, CH4, acetate production, ferulic acid and p-coumaric acid releases. The Neocallimastix frontalis Yak16 with M.ruminantium co-culture presented the strongest lignocellulose degradation ability among 20 co-cultures. ConclusionsTwenty fungus-methanogen co-cultures were obtained from the rumen of grazing yaks. The N.frontalis with M.ruminantium co-cultures were highly effective combination for developing a fermentative system that bioconverts lignocellulose to high activity fibre-degrading enzyme, CH4 and acetate. Significance and Impact of the StudyThe N.frontalis with M.ruminantium co-cultures from yaks grazing on the Qinghai-Tibetan Plateau present great potential in lignocellulose biodegradation industry.
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