Efficient enzymatic production of hydroxy fatty acids by linoleic acid 9 hydratase from Lactobacillus plantarum AKU 1009a

AimsThis study aims to produce hydroxy fatty acids efficiently. Methods and ResultsEscherichia coli overexpressing linoleic acid 9 hydratase from Lactobacillus plantarum AKU 1009a was employed to produce hydroxy fatty acids with industrial potential. We found that 280gl(-1) of linoleic acid (1moll(-1)) was converted into (S)-10-hydoxy-cis-12-octadecenoic acid (HYA) with a high conversion rate of 98% (mol/mol) and more than 999% enantiomeric excess (e.e.) by recombinant E.coli cells in the presence of FAD and NADH. In the same way, many kinds of C18 unsaturated fatty acids with 9 carbon double bond (280gl(-1)) were converted into corresponding 10-hydroxy fatty acids with the conversion rates over 95% (mol/mol). We also produced HYA at a high rate of accumulation (289gl(-1)) with a high yield (97mol%) in a reaction mixture that contained glucose instead of NADH. ConclusionsWe developed a process for producing several types of hydroxy fatty acids with high accumulation rates and high yields. Significance and Impact of the StudyHydroxy fatty acids are important materials for the chemical, food, cosmetic and pharmaceutical industries, and thus they have recently attracted much interest in a variety of research fields. However, the mass production of hydroxy fatty acids has been limited. This method of hydroxy fatty acids production will facilitate the widespread application of hydroxy fatty acids in various industries.