Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 120, Issue 2, Pages 289-300Publisher
WILEY
DOI: 10.1111/jam.13030
Keywords
amphotericin B; anti-biofilm; bacillomycin D; Candida albicans; synergy; wound healing
Categories
Funding
- Sapienza Universita di Roma
Ask authors/readers for more resources
AimsIn this study, we evaluated the ability of the lipopeptide bacillomycin D and the antifungal drug amphotericin B as well as their combination, to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cell migration. Methods and ResultsThe antibiofilm activity of bacillomycin D and its combination with amphotericin B was carried out by crystal violet colorimetric method. Our results have shown that, when combined together at low concentrations nontoxic to mammalian cells, corresponding to 1/32 MIC (039gml(-1)) and 1/4 MIC (006gml(-1)) for bacillomycin D and amphotericin B, respectively, a clear antibiofilm activity is manifested (95% inhibition of biofilm formation) along with a clear inhibition of germ tube formation. Moreover, the effect of both drugs on preformed biofilm of C.albicans strain was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The combination of the two antifungal compounds at 039 and 1gml(-1) for bacillomycin D and amphotericin B, respectively, resulted in a clear enhancement of biofilm eradication compared to the results obtained with each drug alone. Furthermore, this combination was found to promote the closure of a gap produced in a monolayer of human keratinocytes. ConclusionsBacillomycin D and its combination with amphotericin B display impressive anti-biofilm and wound-healing activities. Significance and Impact of the StudyApplication of the lipopeptide bacillomycin D and the antifungal drug amphotericin B in medical devices may offer a promising alternative for topical treatment of Candida-associated infections in the setting of a wound.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available