Journal
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
Volume 27, Issue 3, Pages 357-371Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10837450.2022.2055569
Keywords
Nanotechnology therapy; nucleus targeting; drug delivery; pH; enzyme dual sensitive; poly(amidoamine)
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Funding
- National Natural Science Foundation of China [81541060]
- Science and Technology Projects from the Science Technology and Innovation Committee of Shenzhen Municipality [JCJY20170818110340383]
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In this study, a pH/enzyme dual sensitive nanocarrier was developed for direct drug delivery into the nucleus. The nanocarrier demonstrated controlled drug release and exhibited promising anticancer effects both in vitro and in vivo.
Direct delivery of drugs into the nucleus is a promising nanotechnology therapy, since the nucleus is one of the most important organelles controlling cell proliferation and apoptosis. Here, we report a nucleus-targeting nanocarrier for nuclear drug delivery using a pH/enzyme dual sensitive strategy. The specific ligand PGM (PKKKRKV-GFLG-Mp), composed of nuclear localization sequence (PKKKRKV), enzyme-sensitive tetrapeptide (Gly-Phe-Leu-Gly, GFLG), and pH-sensitive molecules morpholine (Mp), was modified on poly (amidoamine) (PAMAM) by maleimide active polyethylene glycol ester (NHS-PEG-MAL) to form PAMAM-PEG-PGM. Doxorubicin (DOX) was loaded into the cavity of PAMAM to prepare DOX/PAMAM-PEG-PGM. In vitro release study suggested DOX release from DOX/PAMAM-PEG-PGM nanoparticles followed pH and enzyme-triggered manner. In vitro studies showed DOX/PAMAM-PEG-PGM nanoparticles could not only promote cell internalization through the charge switching of morpholine, but also achieve nuclear internalization by the mediation of composite formed by NLS and importin alpha/beta receptor. Further, employing H22 tumour-bearing BALB/c mice as a model, the systemic distribution and anticancer effects of nanoparticles were studied in vivo. The results indicated the nanoparticles could preferentially accumulate in the tumour site in vivo, and the tumour inhibition rate was 88.47%. In short, the nanoparticles developed could be promising in application to nucleus-targeting therapy to enhance antitumour activity.
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