Journal
PHARMACEUTICAL BIOLOGY
Volume 60, Issue 1, Pages 909-914Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2022.2066139
Keywords
Lung cancer; breast cancer; traditional Chinese medicine
Funding
- Scientific Research Projects for Basic Scientific Research in Heilongjiang Provincial Universities [2020KYYWF-0802]
- Natural Science Foundation of Heilongjiang Province [LH2019H062]
- Applied Technology Research and Development Programme in Mudanjiang [HT2020NS085]
- Doctoral Scientific Research Foundation of Mudanjiang Medical University
- Scientific Research Projects for Basic Scientific Research in Heilongjiang Provincial Universities
Ask authors/readers for more resources
This study evaluated the effects of red ginseng polysaccharide (RGP) in cancer cells, finding that RGP inhibited proliferation and induced ferroptosis in lung and breast cancer cells by targeting GPX4.
Context Red ginseng polysaccharide (RGP) is an active component of the widely used medicinal plant Panax ginseng C. A. Meyer (Araliaceae), which has displayed promising activities against cancer cells. However, the detailed molecular mechanism of RGP in ferroptosis is still unknown. Objective This study evaluates the effects of RGP in cancer cells. Materials and methods A549 and MDA-MB-231 cells were used. Cell proliferation was measured by CCK-8 assay after being treated with RGP at concentrations of 0, 50, 100, 200, 400, 800 and 1600 mu g/mL at 0, 12, 24 and 48 h. Lipid reactive oxygen species (ROS) levels were assessed by C11-BODIPY assay. The control group was treated with PBS. Results RGP inhibited human A549 (IC50: 376.2 mu g/mL) or MDA-MB-231(IC50: 311.3 mu g/mL) proliferation and induced lactate dehydrogenase (LDH) release, promoted ferroptosis and suppressed the expression of GPX4. Moreover, the effects of RGP were enhanced by the ferroptosis inducer erastin, while abolished by ferroptosis inhibitor ferrostatin-1. Discussion and conclusions Our study is the first to demonstrate (1) the anticancer activity of RGP in human lung cancer and breast cancer. (2) RGP presented the anti-ferroptosis effects in lung and breast cancer cells via targeting GPX4.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available