Journal
PHARMACEUTICAL BIOLOGY
Volume 60, Issue 1, Pages 1055-1062Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2022.2074465
Keywords
Traditional Chinese medicine; hyperhidrosis; pharmacodynamics; metabolic mechanism
Funding
- Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0405]
- National Natural Science Foundation of China [81973433]
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This study found that Mulisan decoction has a good antiperspirant effect and can improve metabolism. These findings provide insights for further clinical research on immune improvement and antiperspirant effects.
Context Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear. Objective To investigate the antiperspirant effect and underlying mechanisms of MLS. Materials and methods Fifty rats were divided into control, model, and three doses of MLS intervention groups (n = 10). Rats except for control group were induced diseases features of the applicable scope of MLS via i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats via s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-gamma, and TNF-alpha. Rat plasma was collected for endogenous metabolite analysis via UPLC-QE-Focus-MS. Results Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio via increasing IL-2 (38.3%), IFN-gamma (20.1%), and TNF-alpha (22.0%) and decreasing IL-6 (24.7%) compared with the model group (p < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS (p < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism. Conclusions MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect.
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