Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 183, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2022.105084
Keywords
Glutathione S-transferase; Insecticide detoxification; Locusta migratoria; Malathion; Recombinant enzyme
Categories
Funding
- National Natural Science Foundation of China [30810103907, 31601697, 31101463]
- Shanxi Scholarship Council of China [2021-019]
- Doctor' s Research Fund of Shanxi Academy of Agricultural Sciences [YBSJJ1704]
- Programs of Applied Basic Research of Shanxi Province [201601D202058]
- Shanxi Province Science Foundation for Excellent Youth [201901D211194]
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This study investigated the ability of two GSTEs to metabolize insecticides in the locust. One of the GSTEs was found to be able to metabolize malathion and DDT, and participate in the detoxification of the acetylcholinesterase inhibitor malathion. These findings are important for understanding insecticide resistance mechanisms and managing insect populations in the field.
The insect-specific epsilon class of glutathione S-transferases (GSTEs) plays important roles in insecticide detoxification in insects. In our previous work, five GSTEs were identified in Locusta migratoria, and two recombinant GSTEs, rLmGSTE1 and rLmGSTE4, showed high catalytic activity when 1-chloro-2,4-dinitrobenzene (CDNB) was used as a substrate. In this work, we further investigated whether these two GSTEs could metabolize three insecticides including malathion, deltamethrin and DDT. Using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC/MS) method, we found that rLmGSTE4, but not rLmGSTE1, can metabolize malathion and DDT. Malathion bioassays of L. migratoria after the expression of LmGSTE4 was suppressed by RNA interference (RNAi) showed increased insect mortality from 33.8% to 68.9%. However, no changes in mortality were observed in deltamethrin- or DDT-treated L. migratoria after the expression of LmGSTE4 was suppressed by RNAi. Our results provided direct evidences that LmGSTE4 participates in malathion detoxification in L. migratoria. These findings are important for understanding the mechanisms of insecticide resistance in L. migratoria and developing new strategies for managing the insect populations in the field.
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