Journal
PARASITOLOGY INTERNATIONAL
Volume 88, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2022.102541
Keywords
Plasmodium falciparum; Malaria; Drug resistance; School-age children; Democratic Republic of Congo
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Funding
- Japan International Cooperation Agency
- Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine, Nagasaki University [2020-Ippan-14, 2020-Ippan-23]
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This study found a low prevalence of genetic markers associated with chloroquine and sulfadoxine-pyrimethamine resistance in school-age children in Kinshasa. It supports the continued use of ACTs and IPTp-SP.
Background: The emergence and spread of Plasmodium falciparum parasites resistant to antimalarial drugs constitutes an obstacle to malaria control and elimination. This study aimed to identify the prevalence of polymorphisms in pfk13, pfmdr1, pfdhfr, pfdhps and pfcrt genes in isolates from asymptomatic and symptomatic school-age children in Kinshasa. Methods: Nested-PCR followed by sequencing was performed for the detection of pfk13, pfmdr1, pfdhfr, pfdhps and pfcrt polymorphisms. Results: Two mutations in pfk13, C532S and Q613E were identified in the Democratic Republic of Congo for the first time. The prevalence of the drug-resistance associated mutations pfcrt K76T, pfdhps K540E and pfmdr1 N86Y was low, being 27%, 20% and 9%, respectively. Conclusion: We found a low prevalence of genetic markers associated with chloroquine and sulfadoxinepyrimethamine resistance in Kinshasa. Furthermore, no mutations previously associated with resistance against artemisinin and its derivatives were observed in the pfK13 gene. These findings support the continued use of ACTs and IPTp-SP. Continuous molecular monitoring of antimalarial resistance markers is recommended.
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