4.8 Article

Genome Mining and Metabolomics Unveil Pseudonochelin: A Siderophore Containing 5-Aminosalicylate from a Marine-Derived Pseudonocardia sp. Bacterium

Journal

ORGANIC LETTERS
Volume 24, Issue 22, Pages 3998-4002

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.2c01408

Keywords

-

Funding

  1. University of Wisconsin-Madison School of Pharmacy
  2. NIH through the administration of NIAID [U19AI109673, U19AI142720]
  3. University of Wisconsin Carbone Cancer Center Support Grant [P30 CA014520]
  4. UW2020 grant from the Wisconsin Alumni Research Foundation
  5. NIH [P41GM103399]

Ask authors/readers for more resources

In this study, a novel marine-derived siderophore, Pseudonochelin (1), was discovered using genome mining and metabolomics technologies. It was found that 1 contained a previously unidentified 5-aminosalicylic acid unit. Through annotation of a putative biosynthetic gene cluster and bioinformatics analysis, the biosynthesis of 1 was proposed. Furthermore, 1 exhibited iron-dependent antibacterial activity both in vitro and in vivo.
Pseudonochelin (1), a siderophore from a marine-derived Pseudonocardia sp. bacterium, was discovered using genome mining and metabolomics technologies. A 5-aminosalicylic acid (5-ASA) unit, not previously found in siderophore natural products, was identified in 1. Annotation of a putative psn biosynthetic gene cluster combined with bioinformatics and isotopic enrichment studies enabled us to propose the biosynthesis of 1. Moreover, 1 was found to display in vitro and in vivo antibacterial activity in an iron-dependent fashion.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available