Genome Mining and Metabolomics Unveil Pseudonochelin: A Siderophore Containing 5-Aminosalicylate from a Marine-Derived Pseudonocardia sp. Bacterium

Pseudonochelin (1), a siderophore from a marine-derived Pseudonocardia sp. bacterium, was discovered using genome mining and metabolomics technologies. A 5-aminosalicylic acid (5-ASA) unit, not previously found in siderophore natural products, was identified in 1. Annotation of a putative psn biosynthetic gene cluster combined with bioinformatics and isotopic enrichment studies enabled us to propose the biosynthesis of 1. Moreover, 1 was found to display in vitro and in vivo antibacterial activity in an iron-dependent fashion.

Automated summary

In this study, a novel marine-derived siderophore, Pseudonochelin (1), was discovered using genome mining and metabolomics technologies. It was found that 1 contained a previously unidentified 5-aminosalicylic acid unit. Through annotation of a putative biosynthetic gene cluster and bioinformatics analysis, the biosynthesis of 1 was proposed. Furthermore, 1 exhibited iron-dependent antibacterial activity both in vitro and in vivo.