Journal
OPTICS LETTERS
Volume 47, Issue 9, Pages 2306-2309Publisher
Optica Publishing Group
DOI: 10.1364/OL.449400
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Funding
- National Natural Science Foundation of China [61927810, 62005219]
- Fundamental Research Funds for the Central Universities [310202011qd004]
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In this study, a dual-wavelength surface plasmon resonance holographic microscopy (SPRHM) is proposed to measure the cell-substrate distance and cytoplasm refractive index simultaneously. The method utilizes phase-contrast surface plasmon resonance (SPR) images to demodulate the distributions of cell-substrate distance and cytoplasm refractive index. Experimental measurements on MDA-MB-231 cells and IDG-SW3 cells validate the feasibility of this approach, which provides a valuable tool in biological research.
Studying the basic characteristics of living cells is of great significance in biological research. Bio-physical parameters, including cell-substrate distance and cytoplasm refractive index (RI), can be used to reveal cellular properties. In this Letter, we propose a dual-wavelength surface plasmon resonance holographic microscopy (SPRHM) to simultaneously measure the cell-substrate distance and cytoplasm RI of live cells in a wide-field and non-intrusive manner. Phase-contrast surface plasmon resonance (SPR) images of individual cells at wavelengths of 632.8 nm and 690 nm are obtained using an optical system. The two-dimensional distributions of cell-substrate distance and cytoplasm RI are then demodulated from the phase-contrast SPR images of the cells. MDA-MB-231 cells and IDG-SW3 cells are experimentally measured to verify the feasibility of this approach. Our method provides a useful tool in biological fields for dual-parameter detection and characterization of live cells. (C) 2022 Optica Publishing Group
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