Journal
OPTICS LETTERS
Volume 47, Issue 11, Pages 2618-2621Publisher
Optica Publishing Group
DOI: 10.1364/OL.457144
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Funding
- European Research Council [692726 GlobalBioIm]
- Haute ecole Specialisee de Suisse Occidentale
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Optical projection tomography (OPT) is a powerful tool for 3D imaging of mesoscopic samples. This study focuses on addressing the issue of refractive-index mismatch and proposes a fast and efficient reconstruction method to correct the induced artifacts.
Optical projection tomography (OPT) is a powerful tool for three-dimensional (3D) imaging of mesoscopic samples. While it is able to achieve resolution of a few tens of microns over a sample volume of several cubic centimeters, the reconstructed images often suffer from artifacts caused by inaccurate calibration. In this work, we focus on the refractive-index mismatch between the sample and the surrounding medium. We derive a 3D cone-beam forward model of OPT that approximates the effect of refractive-index mismatch. We then implement a fast and efficient reconstruction method to correct for the induced seagull-shaped artifacts on experimental images of fluorescent beads. (C) 2022 Optica Publishing Group
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