Precocious Mexican fruit fly methoprene-fed males inhibit female receptivity and perform sexually as mature males

The sterile insect technique (SIT) has been used successfully for the control of fruit flies. The efficiency of this technique can be significantly reduced when sterile released insects are exposed to adverse conditions and predators, as a great number of sterile insects die before reaching sexual maturity and thus fail to mate with wild females. Treatments with juvenile hormone (JH) analogues such as methoprene (M) significantly reduce the time to reach sexual maturity by sterile Anastrepha ludens (Loew) (Diptera: Tephritidae) males. In this study, we compared the sexual performance of non-treated sexually mature males with young males that had been sexually accelerated with M. Furthermore, we compared the ability of M-fed males in inhibiting female remating compared with sexually mature males. Results showed that at 5days M-fed males had lower mating success than mature males; however, 6-day-old (0.1%) M-fed males had the same amount of matings as mature 13-day-old males. Young 5- to 10-day-old M-fed males also had similar number of matings as mature non-treated 12- to 17-day-old males. There were no differences in copula duration between treatments. Moreover, there were no differences between the fertility, fecundity or refractory period of females mated with either young male fed M or normal sexually mature males. These results indicated that young males that were sexually accelerated with M have the same sexual performance as non-treated sexually mature males. Implications of using M as a pre-release treatment for A.ludens controlled through SIT are discussed.