Journal
NATURE CELL BIOLOGY
Volume 24, Issue 4, Pages 554-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41556-022-00877-0
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Funding
- MIT Stem Cell Initiative
- Breast Cancer Research Foundation
- Advanced Medical Research Foundation
- National Cancer Institute Program [R01-CA078461, R35-CA220487]
- Susan G. Komen Postdoctoral Fellowship [PDF15301255]
- American Cancer Society-New England Division-Ellison Foundation Postdoctoral Fellowship [PF-15-131-01-CSM]
- Ludwig Center for Molecular Oncology at MIT
- David H. Koch Graduate Fellowship
- Scientific and Technological Research Council of Turkey (TUBITAK) [216S461]
- Koc University Research Center for Translational Medicine (KUTTAM) - Presidency of Strategy and Budget of Turkey
- Ludwig Center for Molecular Oncology
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Loss of PRC2 or KMT2D-COMPASS enables distinct epithelial-mesenchymal transition (EMT) trajectories in carcinoma cells, with differing effects on metastatic ability. These findings provide insight into how carcinoma cells control their entrance into and residence in different states, and which states are favorable for metastasis.
Through genome-wide and focused CRISPR screens, Zhang et al. discover that loss of PRC2 or KMT2D-COMPASS enables distinct EMT trajectories, which exert differential effects on the metastatic capability of carcinoma cells. Epithelial-mesenchymal transition (EMT) programs operate within carcinoma cells, where they generate phenotypes associated with malignant progression. In their various manifestations, EMT programs enable epithelial cells to enter into a series of intermediate states arrayed along the E-M phenotypic spectrum. At present, we lack a coherent understanding of how carcinoma cells control their entrance into and continued residence in these various states, and which of these states favour the process of metastasis. Here we characterize a layer of EMT-regulating machinery that governs E-M plasticity (EMP). This machinery consists of two chromatin-modifying complexes, PRC2 and KMT2D-COMPASS, which operate as critical regulators to maintain a stable epithelial state. Interestingly, loss of these two complexes unlocks two distinct EMT trajectories. Dysfunction of PRC2, but not KMT2D-COMPASS, yields a quasi-mesenchymal state that is associated with highly metastatic capabilities and poor survival of patients with breast cancer, suggesting that great caution should be applied when PRC2 inhibitors are evaluated clinically in certain patient cohorts. These observations identify epigenetic factors that regulate EMP, determine specific intermediate EMT states and, as a direct consequence, govern the metastatic ability of carcinoma cells.
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