Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in similar to 4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.

Automated summary

FLASH-seq (FS) is a full-length single-cell RNA sequencing method that offers increased sensitivity and reduced hands-on time compared to Smart-seq3. It can be easily automated and miniaturized to decrease resource consumption.