Journal
NATURE BIOTECHNOLOGY
Volume 40, Issue 7, Pages 1030-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41587-022-01210-8
Keywords
-
Categories
Funding
- Ludwig Center at Harvard
- Harvard Medical School Epigenetics & Gene Dynamics Initiative
- National Institutes of Health (NIH) R00 Award [CA218832]
- Gilead Sciences
- Bertarelli Rare Cancers Fund
- Glenn Foundation for Medical Research
- American Federation for Aging Research (AFAR) Grant
- American Brain Tumor Association Basic Research Fellowship
- UK Brain Tumour Charities Future Leaders Award [GN-000701]
- Stanford Science Fellowship
- Parker Scholar award
- NIH [U01CA260852, R01 DK103794]
- Cancer Research Institute Technology Impact Award
- Pew-Stewart Scholars for Cancer Research Award
- German Research Foundation [LU 2336/2-1]
- National Cancer Institute [P30-CA14051]
- Koch Institute-Dana-Farber/Harvard Cancer Center Bridge Project
- Food Allergy Science Initiative at the Broad Institute
- New York Stem Cell Foundation
Ask authors/readers for more resources
The combination of single-cell transcriptomics and mitochondrial DNA variant detection is useful for establishing lineage relationships in primary human cells, but current methods are not suitable for analyzing complex tissues. In this study, researchers combined common 3' single-cell RNA-sequencing protocols with mitochondrial transcriptome enrichment to significantly increase coverage and enable high-confidence mutation detection. This method successfully identified skewed immune cell expansions in primary human clonal hematopoiesis.
The combination of single-cell transcriptomics with mitochondrial DNA variant detection can be used to establish lineage relationships in primary human cells, but current methods are not scalable to interrogate complex tissues. Here, we combine common 3' single-cell RNA-sequencing protocols with mitochondrial transcriptome enrichment to increase coverage by more than 50-fold, enabling high-confidence mutation detection. The method successfully identifies skewed immune-cell expansions in primary human clonal hematopoiesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available