Journal
NATURE BIOTECHNOLOGY
Volume 40, Issue 9, Pages 1403-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41587-022-01281-7
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [31788103, 32122064]
- Chinese Academy of Sciences [XDA24030504]
- Hainan Excellent Talent Team
Ask authors/readers for more resources
In this study, a cis-regulatory region in rice was identified through a tiling-deletion-based CRISPR-Cas9 screen, resolving the tradeoff between traits and enhancing grain yield per plant.
Crop genetic improvement requires balancing complex tradeoffs caused by gene pleiotropy and linkage drags, as exemplified by IPA1 (Ideal Plant Architecture 1), a typical pleiotropic gene in rice that increases grains per panicle but reduces tillers. In this study, we identified a 54-base pair cis-regulatory region in IPA1 via a tiling-deletion-based CRISPR-Cas9 screen that, when deleted, resolves the tradeoff between grains per panicle and tiller number, leading to substantially enhanced grain yield per plant. Mechanistic studies revealed that the deleted fragment is a target site for the transcription factor An-1 to repress IPA1 expression in panicles and roots. Targeting gene regulatory regions should help dissect tradeoff effects and provide a rich source of targets for breeding complementary beneficial traits.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available