Journal
NANO LETTERS
Volume 22, Issue 6, Pages 2194-2201Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.1c03691
Keywords
super-resolution fluorescence microscopy; stochastic optical fluctuation imaging; dynamic speckle illumination; near-field speckle patterns
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Funding
- National Research Foundation of the Korean government [2019R1A2B5B03070642, 2020M3E4A1080112, 2017R1A6A3A11036019, 2015R1A3A2066550]
- KAIST EndRun Program - Ministry of Science and ICT [N11170044]
- Samsung Science and Technology Foundation [SSTF-BA1602-05]
- National Research Foundation of Korea [2019R1A2B5B03070642, 2020M3E4A1080112, 2017R1A6A3A11036019] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study presents a surface-sensitive super-resolution technique utilizing dynamic near-field speckle illumination, which enables the full super-resolving power of SOFI. Computational and experimental results demonstrate significant enhancements in spatial resolution, with the potential to achieve sub-100 nm resolution.
Stochastic optical fluctuation imaging (SOFI) generates super-resolution fluorescence images by emphasizing the positions of fluorescent emitters via statistical analysis of their on-and-off blinking dynamics. In SOFI with speckle illumination (S-SOFI), the diffraction-limited grain size of the far-field speckles prevents independent blinking of closely located emitters, becoming a hurdle to realize the full super-resolution granted by SOFI processing. Here, we present a surface-sensitive super-resolution technique exploiting dynamic near-field speckle illumination to bring forth the full super-resolving power of SOFI without blinking fluorophores. With our near-field SSOFI technique, up to 2.8- and 2.3-fold enhancements in lateral spatial resolution are demonstrated with computational and experimental fluorescent test targets labeled with conventional fluorophores, respectively. Fluorescent beads separated by 175 nm are also super-resolved by near-field speckles of 150 nm grain size, promising sub-100 nm resolution with speckle patterns of much smaller grain size.
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