Journal
MOLECULES
Volume 27, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/molecules27092779
Keywords
Nigella sativa oil; thymoquinone; epigenetic; cancer; UHRF1; DNMT1; HDAC1
Funding
- Institutional Fund Projects [IFPRC083-130-2020]
- King Abdulaziz University Jeddah, Saudi Arabia
- Ministry of Education, Saudi Arabia
Ask authors/readers for more resources
Nigella sativa oil, commonly known as black seed oil (BSO), contains the active compound thymoquinone (TQ) which inhibits cell proliferation and induces apoptosis by targeting epigenetic factors and proteins. This study compared the effects of BSO and pure TQ on cancer cells and found that both had similar inhibitory and apoptotic effects. Molecular docking and simulation showed that TQ had good binding affinity to UHRF1 and HDAC1, suggesting it as a promising strategy for epigenetic therapy.
Nigella sativa oil, commonly known as black seed oil (BSO), is a well-known Mediterranean food, and its consumption is associated with beneficial effects on human health. A large number of BSO's therapeutic properties is attributed to its pharmacologically active compound, thymoquinone (TQ), which inhibits cell proliferation and induces apoptosis by targeting several epigenetic players, including the ubiquitin-like, containing plant homeodomain (PHD) and an interesting new gene, RING finger domains 1 (UHRF1), and its partners, DNA methyltransferase 1 (DNMT1) and histone deacetylase 1 (HDAC1). This study was designed to compare the effects of locally sourced BSO with those of pure TQ on the expression of the epigenetic complex UHRF1/DNMT1/HDAC1 and the related events in several cancer cells. The gas chromatographs obtained from GC-MS analyses of extracted BSO showed that TQ was the major volatile compound. BSO significantly inhibited the proliferation of MCF-7, HeLa and Jurkat cells in a dose-dependent manner, and it induced apoptosis in these cell lines. BSO-induced inhibitory effects were associated with a significant decrease in mRNA expression of UHRF1, DNMT1 and HDAC1. Molecular docking and MD simulation showed that TQ had good binding affinity to UHRF1 and HDAC1. Of note, TQ formed a stable metal coordinate bond with zinc tom, found in the active site of the HDAC1 protein. These findings suggest that the use of TQ-rich BSO represents a promising strategy for epigenetic therapy for both solid and blood tumors through direct targeting of the trimeric epigenetic complex UHRF1/DNMT1/ HDAC1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available