4.6 Article

Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells

Related references

Note: Only part of the references are listed.
Article Biotechnology & Applied Microbiology

Engineered pegRNAs improve prime editing efficiency

James W. Nelson et al.

Summary: Prime editing technology enhances editing efficiency by optimizing the structure of pegRNAs, preventing degradation of the 3' region from affecting system activity, without increasing the risk of off-target editing.

NATURE BIOTECHNOLOGY (2022)

Letter Biotechnology & Applied Microbiology

Precise genome modification in tomato using an improved prime editing system

Yuming Lu et al.

PLANT BIOTECHNOLOGY JOURNAL (2021)

Article Biotechnology & Applied Microbiology

High-efficiency prime editing with optimized, paired pegRNAs in plants

Qiupeng Lin et al.

Summary: Designing prime binding sites with a certain melting temperature and using two pegRNAs can substantially enhance the efficiency of prime editing.

NATURE BIOTECHNOLOGY (2021)

Review Plant Sciences

Construct design for CRISPR/Cas-based genome editing in plants

Md Mahmudul Hassan et al.

Summary: CRISPR construct design is essential for genome editing, involving selection of Cas proteins, gRNAs, and regulatory elements. This review covers different options for CRISPR-based genome editors in plant editing, gRNA design, multiplex construct strategies, and challenges in construct optimization.

TRENDS IN PLANT SCIENCE (2021)

Review Plant Sciences

Applications of CRISPR/Cas9-based genome editing in the plant biology

Yingzhuan Zheng et al.

Summary: The CRISPR/Cas9 system has rapidly evolved into a genome editing technology, allowing for specific targeting of DNA sequences by the Cas9 endonuclease and guide RNAs (gRNAs). It is replacing other gene editing technologies and has been applied in plant sciences to improve yield, regulate metabolism, and enhance stress resistance in various plant varieties.

TURKISH JOURNAL OF BOTANY (2021)

Article Biotechnology & Applied Microbiology

Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

Li Wang et al.

Summary: Prime editing is an improved version of the CRISPR-Cas system, utilizing a fusion protein and guide RNA to achieve specified substitutions, insertions, and deletions in the target DNA. Recent studies have demonstrated successful Prime editing in rice and wheat, including significant insertions.

FRONTIERS IN GENOME EDITING (2021)

Letter Biotechnology & Applied Microbiology

Engineering herbicide resistance via prime editing in rice

Haroon Butt et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Letter Biotechnology & Applied Microbiology

Precision genome engineering in rice using prime editing system

Kai Hua et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Letter Biochemistry & Molecular Biology

Plant Prime Editors Enable Precise Gene Editing in Rice Cells

Xu Tang et al.

MOLECULAR PLANT (2020)

Letter Biochemistry & Molecular Biology

Precise Modifications of Both Exogenous and Endogenous Genes in Rice by Prime Editing

Huiyuan Li et al.

MOLECULAR PLANT (2020)

Letter Biochemistry & Molecular Biology

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

Wen Xu et al.

MOLECULAR PLANT (2020)

Article Biotechnology & Applied Microbiology

Prime genome editing in rice and wheat

Qiupeng Lin et al.

NATURE BIOTECHNOLOGY (2020)

Letter Cell Biology

Efficient generation of mouse models with the prime editing system

Yao Liu et al.

CELL DISCOVERY (2020)

Article Biotechnology & Applied Microbiology

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Yuan-Yuan Jiang et al.

GENOME BIOLOGY (2020)

Article Biochemistry & Molecular Biology

Development of Plant Prime-Editing Systems for Precise Genome Editing

Rongfang Xu et al.

PLANT COMMUNICATIONS (2020)

Letter Biotechnology & Applied Microbiology

CRISPResso2 provides accurate and rapid genome editing sequence analysis

Kendell Clement et al.

NATURE BIOTECHNOLOGY (2019)

Review Biochemistry & Molecular Biology

CRISPR-Cas9 System for Genome Engineering of Photosynthetic Microalgae

Vikas Kumar Patel et al.

MOLECULAR BIOTECHNOLOGY (2019)

Article Multidisciplinary Sciences

Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks

Holly A. Rees et al.

NATURE COMMUNICATIONS (2019)

Article Multidisciplinary Sciences

Search-and-replace genome editing without double-strand breaks or donor DNA

Andrew V. Anzalone et al.

NATURE (2019)

Review Biochemistry & Molecular Biology

The Biology of CRISPR-Cas: Backward and Forward

Frank Hille et al.

Article Multidisciplinary Sciences

Transcriptional recording by CRISPR spacer acquisition from RNA

Florian Schmidt et al.

NATURE (2018)

Article Plant Sciences

Precision genome engineering through adenine base editing in plants

Beum-Chang Kang et al.

NATURE PLANTS (2018)

Article Multidisciplinary Sciences

Programmable base editing of A.T to G.C in genomic DNA without DNA cleavage

Nicole M. Gaudelli et al.

NATURE (2017)

Article Multidisciplinary Sciences

Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector

Ziqing Liu et al.

SCIENTIFIC REPORTS (2017)

Article Multidisciplinary Sciences

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Alexis C. Komor et al.

NATURE (2016)

Article Biochemical Research Methods

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

Taek Soon Lee et al.

JOURNAL OF BIOLOGICAL ENGINEERING (2011)

Article Biochemical Research Methods

Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

Sang-Dong Yoo et al.

NATURE PROTOCOLS (2007)