Design, Synthesis, and Antifungal Activity of 4-Amino Coumarin Based Derivatives

A series of 30 succinate dehydrogenase inhibitors (SDHIs) of 4-amino coumarin-based derivatives were designed and synthesized. According to the analysis of fungicidal activity in vitro, most of the compounds expressed broad-spectrum antifungal activity against four plant pathogenic fungi (Alternaria alternata, Alternaria solani, Fusarium oxysporum, and Botrytis cinerea) using the mycelium growth inhibition method. The results showed that compounds 3n with the group of 2-ene-3-methyl-butyl and 4e with the group of 2-bromo-1-oxo-hexyl displayed excellent activity against Alternaria alternata and Alternaria solani, with EC50 values of 92145 mu g/mL. Molecular docking showed that the inhibitor 3n was completely locked into the cavity of SDH, forming a conventional hydrogen bond interacting with the amino acid residue TYR58. The present work indicates that these derivatives would serve as novel potential fungicides targeting SDH.

Automated summary

A series of 4-amino coumarin-based succinate dehydrogenase inhibitors (SDHIs) were designed and synthesized. These compounds showed broad-spectrum antifungal activity against various plant pathogenic fungi in vitro. Among them, compounds 3n and 4e displayed excellent activity against Alternaria alternata and Alternaria solani.