Deeper Insight into the Volatile Profile of Rosa willmottiae with Headspace Solid-Phase Microextraction and GC-MS Analysis

As the distribution center of Rosa in the world, China has abundant wild germplasm resources, which can contribute to the breeding of modern roses. To explore the potential value of wild roses distributed in the Sichuan-Tibet region, solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) were used to determine the volatile organic compounds (VOCs) in Rosa willmottiae flowers at three flowering stages (bud stage, initial flowering stage, full flowering stage). Meanwhile, we compared the VOCs of R. willmottiae with different phenotypes (double flowers and single flowers). A total of 74 volatile compounds were identified. The results show that the essential substances belong to alcohols and terpenoids. The main volatile organic compounds are 2-phenyl ethanol (20.49%), benzyl alcohol (10.69%), beta-maaliene (8.66%), geranyl acetate (8.47%), and (+)-alpha-long pinene (6.127%). Different flowering stages had great influence on the volatile profile, from the bud stage to full flowering stage; the content of terpenoids released decreased by 6.17%, whereas alcohols and esters increased by 8.58% and 11.56%, respectively. The chemical diversity and the content of the main components with a different phenotype were not significantly different. Our result will provide a theoretical basis for the development and utilization of Rosa willmottiae in Sichuan and Tibet.

Automated summary

This study used SPME and GC-MS techniques to analyze the volatile organic compounds in Rosa willmottiae flowers at different flowering stages and phenotypes. A total of 74 volatile compounds were identified. The results showed that different flowering stages had a significant impact on the characteristics of volatile compounds in the flowers, providing a theoretical basis for the development and utilization of Rosa willmottiae in Sichuan and Tibet.