Journal
MOLECULES
Volume 27, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/molecules27092681
Keywords
Thunnas albacares; antioxidant peptide; MPO; molecular docking
Funding
- Key-Area Research and Development Program of Guangdong Province [2020B1111030004]
- Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0406]
- National Key R&D Program of China [2018YFC0311202]
- Key R&D Program of Hainan Province [SQ2021SHFZ0795]
- Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2021PY05]
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Antioxidant peptides from tuna protein were found to effectively defend against oxidative stress damage. The peptide ACGSDGK exhibited better antioxidant activity and inhibition of MPO compared to the reference peptide hLF1-11. Molecular docking analysis showed strong interactions between ACGSDGK and MPO, blocking the catalytic activity of MPO.
Antioxidative peptides that inhibit myeloperoxidase (MPO) enzyme activity can effectively defend against oxidative stress damage. The antioxidant peptides from tuna protein were produced using alcalase hydrolysis and purified by ultrafiltration and Sephadex G-15, and the fractions with the highest free radicals scavenging ability and oxygen radical absorbance capacity (ORAC) values were sequenced using HPLC-MS/MS. Fifty-five peptide sequences were identified, 53 of which were successfully docked into MPO. The representative peptide ACGSDGK had better antioxidant activity and inhibition of MPO chlorination and peroxidation than the reference peptide hLF1-11. The docking model further showed intense molecular interactions between ACGSDGK and MPO, including hydrogen bonds, charge, and salt bridge interactions, which occluded the active site and blocked the catalytic activity of MPO. These results suggested that the antioxidant peptide ACGSDGK has the potential to inhibit oxidative stress and alleviate inflammation in vivo because of its inhibitory effect on the MPO enzyme.
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