Multiplex precision gene editing by a surrogate prime editor in rice

Article Plant Sciences

A design optimized prime editor with expanded scope and capability in plants

Wen Xu et al.

Summary: This study demonstrated that using N-terminal reverse transcriptase-Cas9 nickase fusion and introducing multiple-nucleotide substitutions in the reverse transcriptase template can significantly enhance the efficiency of prime editing in plants and human cells. The optimized approach achieved higher editing frequencies compared to the original editor, Prime Editor 3, showing the potential to make previously non-editable target sites editable and expand the capabilities of prime editing in the future.

NATURE PLANTS (2022)

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Article Biotechnology & Applied Microbiology

Lysine biofortification in rice by modulating feedback inhibition of aspartate kinase and dihydrodipicolinate synthase

Qing-Qing Yang et al.

Summary: Lysine is the main limiting essential amino acid in rice seeds, and through the construction of mutants, this study effectively increased the free lysine content in rice seeds, showing potential in improving the nutritional quality of rice and other cereal grains.

PLANT BIOTECHNOLOGY JOURNAL (2021)

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Article Biotechnology & Applied Microbiology

High-efficiency prime editing with optimized, paired pegRNAs in plants

Qiupeng Lin et al.

Summary: Designing prime binding sites with a certain melting temperature and using two pegRNAs can substantially enhance the efficiency of prime editing.

NATURE BIOTECHNOLOGY (2021)

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Article Plant Sciences

Identification of herbicide resistance OsACC1 mutations via in planta prime-editing-library screening in rice

Rongfang Xu et al.

Summary: A prime-editing-library-mediated saturation mutagenesis method was developed to expand herbicide-resistant mutations in plants efficiently. This method identified 16 types of herbicide-resistance-endowing mutations at conserved residues of OsACC1, demonstrating its advantage over base-editing-mediated mutagenesis.

NATURE PLANTS (2021)

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Letter Biochemistry & Molecular Biology