Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 66, Issue 11, Pages -Publisher
WILEY
DOI: 10.1002/mnfr.202101030
Keywords
branched-chain amino acids; gut microbiota; insulin resistance; Portulaca oleracea L.; type 2 diabetes
Categories
Funding
- National Key Research and Development Program of China [2016YFC0500307-07, 2016YFC0500305-02, 2017YFD0400505]
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Portulaca oleracea L. extracts (PE) improve diabetes-related biochemical abnormalities by modulating gut microbiota composition and branched-chain amino acids (BCAAs) metabolism, as well as protecting intestinal mucosal epithelium.
Scope: Portulaca oleracea L. extracts (PE) show hypoglycemic function, but the precise mechanism remains obscure. This study is designed to investigate the association of the antidiabetes effect of PE with the gut microbiota modulation and BCAAs metabolism. Methods and Results: The Orbitrap LC-MS to Orbitrap Fusion Lumos Tribrid mass spectrometer is employed to analyze the major compounds in PE. The components of the intestinal microflora in diet-induced/STZ-treated diabetic mice are analyzed by high-throughput 16S rRNA genes sequencing. The results show that PE improves blood glucose and insulin level, increases anti-inflammatory cytokine level, lowers serum branched-chain amino acids (BCAAs), and increases serum glutamine level. PE also protects the mucosal epithelium of the colon and cecum from damage. On the impact of gut microbial composition, PE reduces the Firmicutes to Bacteroidetes ratio and the abundance of the Lachnospiraceae_NK4A136_group, Blautia, Ruminiclostridium_9, Dubosiella, and increases the abundance of the Bacteroides, Akkermansia, and Mucisprillum genera. Bacterial functionality prediction indicates PE potentially inhibits bacterial BCAAs biosynthesis, and promotes the tissue-specific expression of BCAAs catabolic enzyme for reducing BCAAs supplementation. Conclusion: These results reveal that PE improving T2D-related biochemical abnormalities is associated not only with gut microbiota modification but also with the tissue-specific expression of BCAAs catabolic enzyme.
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