Journal
MOLECULAR MICROBIOLOGY
Volume 117, Issue 5, Pages 1104-1120Publisher
WILEY
DOI: 10.1111/mmi.14895
Keywords
cholesterol utilization; ESX1 secretion; granuloma formation; lysosomal trafficking; Mycobacterium tuberculosis; transcriptional regulation
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Funding
- Council of Scientific and Industrial Research, India [21(1088)/19/EMR-II]
- Department of Biotechnology, Ministry of Science and Technology, India [BT/PR20242/MED/29/1062/2016]
- Science and Engineering Research Board [SB/S2/JCB-049/2016]
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Rv0081 is a transcriptional regulator in Mycobacterium tuberculosis that plays a crucial role in the survival and multiplication of the bacterium within its host. It regulates the expression of genes involved in cholesterol degradation and import and activates the secretion of EspR protein, which in turn is involved in lysosomal trafficking subversion and granuloma expansion.
The transcriptional network of Mycobacterium tuberculosis is designed to enable the organism to withstand host-associated stresses and to exploit the host milieu for its own survival and multiplication. Rv0081 (MT0088) is a transcriptional regulator whose interplay with other gene regulatory proteins and role in enabling M. tuberculosis to thrive within its host is incompletely understood. M. tuberculosis utilizes cholesterol within the granuloma. We show that deletion of Rv0081 compromises the ability of M. tuberculosis to utilize cholesterol as the sole carbon source, to subvert lysosomal trafficking, and to form granulomas in vitro. Rv0081 downregulates expression of the nucleoid-associated repressor Lsr2, leading to increased expression of the cholesterol catabolism-linked gene kshA and genes of the cholesterol importing operon, accounting for the requirement of Rv0081 in cholesterol utilization. Furthermore, Rv0081 activates EspR which is required for secretion of ESX-1 substrates, which in turn are involved in subversion of lysosomal trafficking of M. tuberculosis and granuloma expansion. These results provide new insight into the role of Rv0081 under conditions which resemble the environment encountered by M. tuberculosis within its host. Rv0081 emerges as a central regulator of genes linked to various pathways which are crucial for the survival of the bacterium in vivo.
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