Journal
MOLECULAR MICROBIOLOGY
Volume 117, Issue 5, Pages 1213-1226Publisher
WILEY
DOI: 10.1111/mmi.14902
Keywords
c-di-GMP; proteome; Pseudomonas aeruginosa; ribosome profiling; RNA-seq
Categories
Funding
- Deutsche Forschungsgemeinschaft [EXC 2155, 390874280, SPP1879]
- European Commission [COMBAT 724290]
- Novo Nordisk Foundation [18OC0033946]
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C-di-GMP signaling directly affects bacterial behavior by influencing protein functionality, and it can also have a global impact on gene transcription or translation. This study investigated the effects of changes in intracellular c-di-GMP levels on gene expression and protein production in Pseudomonas aeruginosa, and found that an increase in c-di-GMP levels caused a switch in phenotype before any changes in gene transcription or protein abundance were observed.
C-di-GMP signaling can directly influence bacterial behavior by affecting the functionality of c-di-GMP-binding proteins. In addition, c-di-GMP can exert a global effect on gene transcription or translation, for example, via riboswitches or by binding to transcription factors. In this study, we investigated the effects of changes in intracellular c-di-GMP levels on gene expression and protein production in the opportunistic pathogen Pseudomonas aeruginosa. We induced c-di-GMP production via an ectopically introduced diguanylate cyclase and recorded the transcriptional, translational as well as proteomic profile of the cells. We demonstrate that rising levels of c-di-GMP under growth conditions otherwise characterized by low c-di-GMP levels caused a switch to a non-motile, auto-aggregative P. aeruginosa phenotype. This phenotypic switch became apparent before any c-di-GMP-dependent role on transcription, translation, or protein abundance was observed. Our results suggest that rising global c-di-GMP pools first affects the motility phenotype of P. aeruginosa by altering protein functionality and only then global gene transcription.
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