Journal
MOLECULAR CELL
Volume 82, Issue 6, Pages 1083-1085Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2022.02.024
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Funding
- NIH [R01 GM099604]
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In this paper, the authors present a successful structure-guided approach to improve the genome-editing efficiency of CRISPR-CasX in Deltaproteobacteria and Planctomycetes.
In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by-10-20 fold in vitro and mean-editing efficiency by-2-3 fold in human cells.
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