Journal
MOLECULAR BIOLOGY AND EVOLUTION
Volume 39, Issue 5, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msac103
Keywords
segmental duplication; retrotransposon; lcr16a; SVA
Funding
- Deutsche Forschungsgemeinschaft [DFG DA 545/3-1, 491454463]
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This study reveals the role of segmental duplications (SDs) and Alu short interspersed elements (SINEs) in primate genomes. It shows that a core duplicon called lcr16a contributes to the propagation of interspersed SDs in primates, with SVA retrotransposon being an integral component of lcr16a in Asian and African great apes. The findings also suggest that SVA recombination-mediated integration of a circular intermediate is the founding event of a lineage-specific lcr16a expansion in hominines.
Segmental duplications (SDs) constitute a considerable fraction of primate genomes. They contribute to genetic variation and provide raw material for evolution. Groups of SDs are characterized by the presence of shared core duplicons. One of these core duplicons, low copy repeat (lcr)16a, has been shown to be particularly active in the propagation of interspersed SDs in primates. The underlying mechanisms are, however, only partially understood. Alu short interspersed elements (SINEs) are frequently found at breakpoints and have been implicated in the expansion of SDs. Detailed analysis of lcr16a-containing SDs shows that the hominid-specific SVA (SINE-R-VNTR-Alu) retrotransposon is an integral component of the core duplicon in Asian and African great apes. In orang-utan, it provides breakpoints and contributes to both interchromosomal and intrachromosomal lcr16a mobility by inter-element recombination. Furthermore, the data suggest that in hominines (human, chimpanzee, gorilla) SVA recombination-mediated integration of a circular intermediate is the founding event of a lineage-specific lcr16a expansion. One of the hominine lcr16a copies displays large flanking direct repeats, a structural feature shared by other SDs in the human genome. Taken together, the results obtained extend the range of SVAs' contribution to genome evolution from RNA-mediated transduction to DNA-based recombination. In addition, they provide further support for a role of circular intermediates in SD mobilization.
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