Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 477, Issue 6, Pages 1849-1863Publisher
SPRINGER
DOI: 10.1007/s11010-022-04409-0
Keywords
DIA mass spectrometry; Idiopathic epiretinal macular membrane; Rhegmatogenous retinal detachment with choroidal detachment; Rhegmatogenous retinal detachment
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Funding
- Social Development Project of Jiangsu Provincial Science and Technology Department [BE2017627]
- high-end talent project of Wuxi Health Committee
- reserve top talent project of Wuxi Municipal Health Commission [HB2020032]
- youth project of Wuxi Health Committee [Q202038]
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This study focused on identifying the proteins and signal pathways associated with the etiology of rhegmatogenous retinal detachment (RRD) with choroidal detachment (RRDCD) and providing guidance for diagnosis and treatment. Differentially expressed proteins (DEPs) were identified using mass spectrometry and bioinformatics analysis. The results indicated that inflammation and immunity, protein degradation and absorption, cell adhesion molecules (CAMs), the hedgehog signaling pathway, and lipid metabolism were functionally related to RRDCD. This study provides important clues and the theoretical basis for understanding the pathogenesis, progression, and prognosis of ocular fundus disease.
The prognosis of rhegmatogenous retinal detachment (RRD) with choroidal detachment (RRDCD) is often poor and complicated. This study focused on the identification of the characteristic proteins and signal pathways associated with the etiology of RRDCD and to provide guidance for diagnosis and treatment of RRDCD. In this study, vitreous humor samples were obtained from 16 RRDCD patients, 14 with RRD, 12 with idiopathic epiretinal macular membrane (IEMM), and 5 healthy controls from donated corpse eyes. Data-independent acquisition mass spectrometry and bioinformatics analysis were employed to identify differentially expressed proteins (DEPs). In the vitreous humor, 14,842 peptides were identified. Patients with RRDCD had 249 DEPs (93 upregulated and 156 downregulated), with 89 in patients with RRD and 61 in patients with IEMM. Enrichment analysis of the GO and Kyoto Encyclopedia of Genes and Genomes DEP databases indicated functional clusters related to inflammation and immunity, protein degradation and absorption, cell adhesion molecules (CAMs), the hedgehog signaling pathway, and lipid metabolism. Weighted gene co-expression network analysis showed that DEPs with positive co-expression of RRDCD participated in immune-related pathways led by the complement and coagulation cascade, whereas DEPs with negative co-expression of RRDCD participated in protein degradation and absorption, CAMs, and the hedgehog signaling pathway. In summary, our study provides important clues and the theoretical basis for exploring the pathogenesis, progression, and prognosis of ocular fundus disease.
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