Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 85, Issue 8, Pages 3005-3013Publisher
WILEY
DOI: 10.1002/jemt.24149
Keywords
chlorhexidine; fiber post; peracetic acid; scanning electron microscopy
Categories
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2018/11790-6]
Ask authors/readers for more resources
This study evaluated the effect of different irrigation protocols on the formation of chemical smear layer and the incidence of open dentin tubules. The results showed that irrigation with chlorhexidine resulted in the formation of chemical smear layer, while ethanol was ineffective in removing the smear layer. Peracetic acid was found to be more effective in removing the smear layer.
This study evaluated the effect of irrigation protocols using 95% ethanol (ET) or 1% peracetic acid (PA) prior the use of 2% chlorhexidine (CHX) compared to distilled water (DW) on the chemical smear layer (CSL) formation and incidence of open dentin tubules at the apical, medium, and cervical third of the post-space dentin. Scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS) images were used. Forty bovine roots were endodontically treated. After, post-space preparation was performed and the roots were randomized in four groups (n = 10) according to the irrigation protocol: DW, CHX, CHX-ET and CHX-PA. The chemical composition of CSL and the incidence of open dentin tubules at the post-space thirds were evaluated by EDS (500x magnification) and SEM (2000x magnification) images, respectively. Data from chemical composition of CSL were descriptively analyzed, while the incidence of open dentin tubules was evaluated by scores and submitted to Kruskal-Wallis and Dunn test (p = .05). Cl, Bi, and Si were the chemical elements most found over the dentin after the irrigation with CHX and CHX-ET. Moreover, CHX and CHX-ET showed the highest incidence of CSL (p < .05), but without difference between them (p > .05), regardless of the post-space third. DW and CHX-PA showed similar incidence of CSL (p > .05). No difference on the incidence of open dentin tubules was found for any irrigation protocol and post-space third (p > .05). The use of 1% PA prior the post-space irrigation with CHX decrease the incidence of CSL. Research Highlights The post-space irrigation with chlorhexidine results in the formation of chemical smear layer. Ethanol is not capable to remove the chemical smear layer. Peracetic acid is more effective to remove the chemical smear layer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available