Journal
MICROCHIMICA ACTA
Volume 189, Issue 4, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-022-05250-4
Keywords
Dual immunoplatform; BAFF; APRIL; MoS2/MWCNTs; Amperometry; Serum; SLE; CRC
Categories
Funding
- Spanish Ministerio de Ciencia, Innovacion y Universidades [PRE2019-087,596, RTI2018-096,135-B-I00]
- Spanish Ministerio de Ciencia e Innovacion [PID2019-103899RB-I00]
- TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid [S2018/NMT-4349]
- AES-ISCIII program [PI17CIII/00045, PI20CIII/00019]
- Spanish Ministerio de Educacion, Cultura y Deporte
- Flanders Research Foundation (FWO) [1193818 N]
- CRUE-CSIC agreement
- Springer Nature
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A dual immunosensor was developed for the simultaneous determination of two important immunity-related cytokines, BAFF and APRIL. The immunosensor showed high sensitivity and low detection limits, making it suitable for detecting these cytokines in cancer cell lysates and serum samples.
A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary -MoS2/ MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng-mL(-1) for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
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