Journal
MICROCHIMICA ACTA
Volume 189, Issue 3, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-022-05246-0
Keywords
MicroRNA-21; Electrochemiluminescence; Modified glassy carbon electrode; Metal-organic frameworks; Duplex-specific nuclease
Categories
Funding
- National Natural Science Foundation of China [22074119, 21675124, 21375102]
- National Key R&D Program of China [2019YFD1002405]
- Natural Science Basic Research Program of Shaanxi [2020JM-426]
Ask authors/readers for more resources
A novel electrochemiluminescence biosensor was developed for the determination of miRNA-21, utilizing a hybrid luminescent composite as the luminophore and a signal amplification strategy. The biosensor exhibited strong and stable ECL signal, and had a wide linear range and low detection limit for miRNA-21. This biosensor shows promise for clinical diagnosis and disease prognosis analysis.
A novel electrochemiluminescence (ECL) biosensor for the determination of microRNA-21 (miRNA-21) was developed, based on a hybrid luminescent Co-MOF-ABEI/Ti3C2Tx composite as an ECL luminophore combined with a duplex-specific nuclease (DSN)-assisted signal amplification strategy. The synthesized Co-MOF-ABEI/Ti3C2Tx composite carrying N-(4-aminobutyl)-N-ethylisoluminol (ABEI) exhibited strong and stable ECL in the presence of reactive oxygen species (ROS). The ECL biosensor was fabricated by adsorbing Co-MOF-ABEI/Ti3C2Tx onto a glassy carbon electrode and covalently coupling the probe DNA onto the surface of the Co-MOF-ABEI/Ti3C2Tx-modified electrode. In the presence of the target miRNA-21, the DSN selectively cleaved the complementary DNA section (S1) to miRNA-21, resulting in the release of the transduction section (S2) and the reuse of miRNA-21 in the subsequent amplification cycle. The interaction of the stem-loop structure of the probe DNA with the Co-MOF-ABEI/Ti3C2Tx-modified glassy carbon electrode with S2 strands led to the opening of the annular part of the probe DNA. Then, the opened guanine (G)-rich sequences of probe DNA were exposed and folded into a hemin/G-quadruplex DNAzyme in the presence of hemin. The catalysis of H2O2 to ROS by the hemin/G-quadruplex DNAzyme significantly enhanced ECL intensity, and this intensity was logarithmically proportional to the concentration of target miRNA-21 between 0.00001 and 10 nM, having a limit of detection of 3.7 fM. The designed ECL biosensor can detect miRNA-21 extracted from HeLa cells, indicating its promising application in clinical diagnosis and disease prognosis analysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available