Journal
MICROCHEMICAL JOURNAL
Volume 177, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2022.107311
Keywords
Molecularly imprinted polymer; Electrochemical sensor; Prostate-specific antigen; Ferrocene; Poly(o-phenylenediamine-co-o-aminophenol)
Categories
Funding
- National Science, Research and Innovation Fund (NSRF)
- Prince of Songkla University [SCI6505027S]
- Center of Excellence for Innovation in Chemistry (PERCH-CIC)
- Ministry of Higher Education, Science, Research and Innovation
- Center of Excellence for Trace Analysis and Biosensor (TAB-CoE) at Prince of Songkhla University of Thailand
- Science Achievement Scholarship of Thailand
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In this study, an electrochemical sensor based on MIP was developed by modifying a screen-printed carbon electrode with PEDOT:PSS@Fc and a MIP layer. The sensor successfully detected the concentration of PSA.
A molecularly imprinted polymer (MIP) of o-phenylenediamine (o-PD) and o-aminophenol (o-AP), electropolymerized in the presence of prostate specific antigen (PSA), was deposited on a screen-printed carbon electrode (SPCE) modified with a poly(styrenesulfonate) doped poly(3,4-ethylenedioxythiophene) ferrocene composite (PEDOT:PSS@Fc) to create a MIP/PEDOT:PSS@Fc/SPCE electrochemical sensor. The MIP layer was characterized by scanning electron microscopy (SEM). PSA was determined using differential pulse voltammetry by measuring the change in the oxidation peak current of Fc. At the optimal monomer molar ratio, electro-polymerization condition, phosphate buffer pH and rebinding time, the MIP sensor produced a linear response over a concentration range of 1.0 x 10(-7)-1.0 x 10(-4) ng mL(-1) PSA. The detection limit was (8.3 +/- 1.3) x 10(-8) ng mL(-1) and selectivity, reproducibility, reusability over 12 cycles and long-term stability over 7 weeks were good. Using the Langmuir adsorption isotherm, the dissociation constant (K-D) of the MIP sensor was 1.91 x 10(-6) ng mL(-1). PSA levels detected in blood serum with the present sensor were consistent with the data from the electrochemiluminescence immunoassay (P > 0.05).
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