Fabrication of morphine detector based on quartz@Au-layer biosensor

Biosensors analytical devices consist of a diagnostic element with a physical converter that can be very useful for analyzing biomarkers on site. The biosensor oscillator was made from a 20 MHz piezoelectric modified gold nano-layer. The response of this biosensor was based on the frequency change resulting from the effect of morphine on the anti-morphine monoclonal antibody. The level of the biosensor was studied with the FE-SEM and XRD analysis. Also, the comparison of the frequency response of this system at temperatures of 5 degrees C, 25 degrees C, and 45 degrees C shows that this system is stable at different temperatures. This device measured the concentration of morphine in both open and closed modes. The results showed that the device has a limit of detection (LOD) of 0.25 ng.mL- 1 and the limit of quantification (LOQ) of 0.25-2500 ng.mL- 1. The detection of this platform is fast (460 s) with a small volume of sample (1 mu L), which showed that the server is compatible with what is required in clinical measurements.

Automated summary

Biosensors analytical devices, consisting of a diagnostic element with a physical converter, are useful for on-site analysis of biomarkers. The biosensor oscillator in this study was made from a 20 MHz piezoelectric modified gold nano-layer, whose response was based on frequency change caused by the effect of morphine on the anti-morphine monoclonal antibody. The stability of the biosensor at different temperatures was confirmed through FE-SEM and XRD analysis. The device demonstrated fast detection speed and small sample volume, making it compatible with clinical measurements.