Journal
MICROCHEMICAL JOURNAL
Volume 174, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2021.107036
Keywords
CRISPR; Cas system; Pathogens; Nucleic acid amplification; Electrochemical sensors; Nanotechnology
Categories
Funding
- Science and Technology innovation Plan of Shanghai [19391902000]
- Civil-Military Fusion Seed Program [2020-RP04]
- Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai [GWV-10.2-YQ48]
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CRISPR/Cas systems are developed as new biosensing tools for accurate and rapid pathogen detection. Researchers achieve signal amplification for detection by combining nanotechnology and various biosensor technologies, greatly improving the sensitivity and specificity of detection.
Accurate and rapid detection of pathogens is essential for food safety, epidemic prevention, disease diagnosis and environmental monitoring. So far, many CRISPR/Cas systems have been gradually developed as new biosensing tools for biological nucleic acid detection. These effectors are coupled with existing nucleic acid amplification methods to produce sensitive, sequence-specific pathogen identification in multiple field-deployable formats. With the maturity of CRISPR technology, researchers have achieved detection signal amplification by combining nanotechnology and various biosensor technologies, which not only greatly improves the sensitivity and specificity of detection, but also enriches signal output forms. Hence, the classification of CRISPR-Cas systems and the properties of Cas effectors in Class2 are outlined. Furthermore, we also highlight the ways to improve pathogen detection performance based on CRISPR/Cas biosensors. The challenges and directions of this field of CRISPR/ Cas-based biosensors are further discussed.
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