Journal
MICROCHEMICAL JOURNAL
Volume 174, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2021.107035
Keywords
miRNA; Exosome; Tetrahedral probe; DSN enzyme
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miRNA plays a crucial role in cancer development, with challenges in accurate extraction from clinical samples. A novel fluorescence assay proposed utilizes DSN signal amplification for accurate analysis of miRNA, offering stable, sensitive, and label-free advantages.
MicroRNA (miRNA) plays a pivotal role in the regulation of cancer developments and could potentially work as an important biomarker candidate for the early-diagnosis, therapy and prognosis of lung cancer. Accurate and sensitive quantification of miRNA extracted from clinical samples in a stable and label-free way remain a huge challenge. Herein, we propose here a novel fluorescence assay for accurate analysis of miRNA via duplex specific nuclease (DSN) signal amplification. Highlights of the methods could be depicted as: i) a favorable detection sensitivity through the DSN enzyme based signal amplification; ii) fluorescence signal generation in a label-free way based on MG/G-quadruplex complex; iii) a stable signal generation even in complicated experimental conditions based on the tetrahedral structure. Eventually, we believe that this proposed sensitive and specific assay has great potential as a miRNA quantification method for use in biomedical research and clinical diagnosis.
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