4.5 Article

Investigation of leptospiral antigen with immunohistochemical and immunofluorescence methods in cattle kidney

Journal

MICROBIAL PATHOGENESIS
Volume 164, Issue -, Pages -

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2022.105434

Keywords

Kidney; Immunohistochemistry; Immunofluorescence; Leptospira interrogans; Cattle

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In this study, the presence of Leptospira interrogans antigens in naturally infected bovine kidney samples was determined using immunohistochemical and immunofluorescence methods. The antigens were detected in 7.14% of the samples tested by both methods. Despite lower rates compared to previous studies, it is important to recognize that this issue still poses problems for animal health.
In this study, it was aimed to determine the presence of Leptospira interrogans antigens in the kidney samples of naturally infected cattle by immunohistochemical and immunofluorescence methods. 70 bovine kidney samples showing macroscopic lesions were examined by immunohistochemical and immunofluorescence methods. The positivity of Leptospira interrogans antigens was detected in 5 (7.14%) kidney samples examined by both methods. As a result; The presence of Leptospira interrogans antigens detected by IHC and IF staining methods in bovine kidneys where research samples were provided was found at the same rates (7.14%). Although it has low rates compared to previous studies, it has been determined that it is current and creates problems in terms of animal health.

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