Journal
MICROBIAL PATHOGENESIS
Volume 165, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2022.105494
Keywords
Mexico; Bacteriophages; Complete genome; Lytic activity; MDR-UPEC; Phage therapy
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Funding
- Departamento de Fortalecimiento Academico, Programa de Becas Postdoctorales, Universidad Nacional Autonoma de Mexico
- Economy and Knowledge Department of the Catalan Government through Consolidated Research Group
- CERCA program [ICRA-ENV 2017 SGR 1124]
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This study sequenced and characterized the genomes of two lytic bacteriophages, showing their lytic activity against multidrug-resistant Escherichia coli strains and the absence of antibiotic resistance, lysogenic and virulence genes.
In this study, the genomes of two lytic bacteriophages, vB_EcoS-phiEc3 and vB_EcoS-phiEc4, were sequenced and characterized using bioinformatics approaches. Whole-genome analysis showed that both phages belonged to the Kagunavirus genus, Guernseyvirinae subfamily and Siphoviridae family. Moreover, their genomes had 45, 288 bp and 44,540 bp, and G + C content of 48.42% and 50.04%, respectively. The genome of vB_EcoS-phiEc3 harbored 80 protein coding sequences (CDSs), whereas vB_EcoS-phiEc4 harbored 75 CDSs. Among them, 50 CDSs in vB_EcoS-phiEc3 and 44 CDSs in vB_EcoS-phiEc4 were considered as functional genes. Their lytic activity against multidrug-resistant uropathogenic Escherichia coli (UPEC) strains, as well as the absence of antibiotic resistance genes, lysogenic and virulence genes, enable vB_EcoS-phiEc3 and vB_EcoS-phiEc4 as a safe therapy option against UPEC infections.
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