Isolation, identification, and pathogenicity of Lelliottia amnigena causing soft rot of potato tuber in China

Potato (Solanum tuberosum L.) is regarded as the fourth most important food crop because of its economic and nutritional benefits. This crop suffers significant annual losses due to a variety of phytopathogens. Bacterial soft rot disease is one of the most serious diseases that cause significant losses in potato yield all over the world. Therefore, identification of a soft rot pathogen is critical for easy control, as each pathogen has distinct ways of being controlled. Lelliottia amnigena is a subgroup of the genus Enterobacter with many species associated with crop plants, making its classification difficult and complex. Therefore, this study focused on the isolation and identification of a newly L. amnigena from rotten potato tuber obtained from the field after harvest, Lanzhou City, China. Four strains designated as PC2, PC3, PC4 and PC5 were isolated from the same rotting potato tuber. Pathogenicity test showed that strain PC3 induced soft rot symptoms on healthy potato tubers. Koch's postulates were confirmed by re-isolating the strain PC3 in the inoculated tubers. Strain PC3 showed a convex, oval and smooth colony, measuring 0.9-1.3 1.8-3.6 mu m under the microscopic observation. Phylogenetic analysis based on 16S rRNA, rpoB and atpD genes showed that strain PC3 species was 99.44%, 97.24%, and 100%, closely related to L. amnigena with accession numbers 240-a-etp (MN208158.1), FDAARGOS (CP023529.1) and R-6 (MN658356.1), respectively. The bacterial strain (PC3) was deposited in the Genbank with the accession number SUB10508072 PC3 OK447935. To the best of our knowledge, this is the first report of L. amnigena causing soft rot on potato tubers in China.

Automated summary

This study isolated and identified a newly discovered L. amnigena from rotten potato tubers in Lanzhou City, China. The strain was found to induce soft rot disease on potato tubers, and phylogenetic analysis showed its close relationship to L. amnigena strains from other sources. This is the first report of L. amnigena causing soft rot on potato tubers in China.