A novel ultrasensitive biosensor based on NiCo-MOF nanostructure and confined to flexible carbon nanofibers with high-surface skeleton to rapidly detect Helicobacter pylori

Herein, we aim at designing and synthesizing a specific and sensitive aptasensor for Helicobacter pylori (H. pylori), using a modified electrode with an electrospun nano-sized bimetallic NiCo-metal-organic frameworks nanostructure (NiCo-MOF@C) as platform. As a result, owning to the hydrogen bonds, large amounts of aptamer strands can be bound to the NiCo-MOF@C. The detection limits of 1 CFU. ml- 1 and the linearity of 101-107 CFU. ml-1 were obtained. The results of our study show that the proposed impedimetric aptamer-based biosensor has specificity, low detection limit, extended range of linear detection and stability for H. pylori. This new NiCoMOF@C will become an excellent aptamer-based sensors (aptasensors) platform to detect various analytes.

Automated summary

A specific and sensitive aptasensor for Helicobacter pylori (H. pylori) was designed using a modified electrode with a nano-sized bimetallic NiCo-metal-organic frameworks nanostructure (NiCo-MOF@C) as platform. Due to hydrogen bonds, a large amount of aptamer strands could bind to NiCo-MOF@C, resulting in a detection limit of 1 CFU.ml-1 and a linear range of 101-107 CFU.ml-1. The proposed aptamer-based biosensor showed specificity, low detection limit, extended linear detection range, and stability for H. pylori.