Centrifugal spinning of polyvinyl alcohol/sodium alginate-di aldehyde-gelatin based antibacterial nanofibers intended for skin tissue engineering

In this study, three types of nanofibers, i.e. polyvinyl alcohol (PVA), alginate di-aldehyde (ADA)/PVA, and ADAgelatin (GEL)/PVA, were fabricated via centrifugal spinning. The spinability of ADA was improved by mixing with PVA. Furthermore, GEL and sesame oil were added in the ADA solution to control the degradation kinetics and to improve the viscosity of the solution, respectively. Later, the solution was fed to a rotating spinneret (3000 rpm at 250 ?) which led to the fabrication of nanofibers. The developed nanofibers were loaded with copper-silver (Cu-Ag) doped mesoporous bioactive glass nanoparticles (MBGNs) to provide therapeutic effects (antibacterial effect). Scanning electron microscopy images showed that the nanofiber diameters were 139 +/- 62 nm, 258 +/- 42 nm, and 281 +/- 34 nm, for PVA, ADA/PVA, and ADA-GEL/PVA respectively, with web-like morphology. Fourier transform infrared spectroscopy confirmed the presence of chemical bonds attributed to the ADA, GEL, and PVA components. The results of the inhibition halo test confirmed that the nanofibers (ADAGEL/PVA & ADA/PVA) exhibit potent antibacterial effect against Staphylococcus aureus and Escherichia coli.

Automated summary

Nanofibers of different types were fabricated using centrifugal spinning and loaded with copper-silver doped bioactive glass nanoparticles to achieve antibacterial effects.