Exploring the structure of RNA-incorporated PEG/PEI modified silica network

Using the sol-gel method, mesoporous silica (MPS) was successfully hybridized with PEI and PEG polymers and encapsulated RNA oligonucleotides in one pot, at a single step. Infrared, Si-29 NMR spectroscopies, thermal analysis, and zeta-potential measurements were performed to analyze the xero-gels' structure. Results showed that silica did not react with polymers on the Si core. RNA-PEI polyelectrolytes were formed on the surface of MPS-PEI hybrids. Whereas in MPS-PEG-PEI, on one side, PEG was hydrogen-bonded to the molecular water adsorbed on the surrounding RNA, while on the other, it was attached to the PEI bound on the silica surface. The hybrid particles were spherical in shape, with diameters ranging from 120 to 200 nm. They were macroporous with an isoelectric point of 10-11, resulting in positively charged surfaces at physiological pH. These particles show promise as future RNA interference vectors due to their positive surface charge leading to chemical stability in circulation, and cell-penetrating properties.

Automated summary

By using the sol-gel method, mesoporous silica was hybridized with PEI and PEG polymers to encapsulate RNA oligonucleotides, forming hybrid particles with positive surface charge for future RNA interference applications. Results from various analyses showed that the hybrid particles exhibited good chemical stability and cell-penetrating properties, indicating their potential as effective RNA interference vectors.