Journal
MARINE POLLUTION BULLETIN
Volume 179, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2022.113684
Keywords
Photo-induced toxicity; Polycyclic aromatic hydrocarbon (PAH); Deepwater horizon (DWH); RNA sequencing (RNA-Seq); Early life stage (ELS) fish; TUNEL assay
Funding
- Gulf of Mexico Research Initiative (GoMRI) [SA-1520]
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This study is the first to provide a global transcriptomic analysis of UV and oil co-exposure in an ELS fish, revealing the apoptosis and impact on multiple physiological pathways caused by photo-induced PAH toxicity in larval red drum.
Ultraviolet (UV) radiation can significantly increase the toxicity of polycyclic aromatic hydrocarbons (PAHs) in crude oil to early life stage (ELS) fishes through photo-induced /photo-enhanced toxicity. However, little is known about the sub-lethal effects and mechanisms of photo-induced PAH toxicity in ELS fishes. The present study investigated apoptosis and global transcriptomic effects in larval red drum (Sciaenops ocellatus) (24-72 h post-fertilization) following co-exposure to oil (0.29-0.30 mu g/L n-ary sumation PAH50) and UV. Apoptosis was quantified using the TUNEL assay, and transcriptomic effects were assessed using RNA sequencing analysis. Apoptotic fluorescence was greatest in the eyes and skin following 24 and 48 h co-exposure to oil and UV, indicating photoinduced toxicity. Consistent with these phenotypic responses, pathways associated with phototransduction, eye development, and dermatological disease were among the top predicted pathways impacted. The present study is the first to provide global transcriptomic analysis of UV and oil co-exposure in an ELS fish.
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