Journal
MARINE DRUGS
Volume 20, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/md20050288
Keywords
keto-deoxy-sugar; 2-keto-3-deoxy sugar acid; 2-keto-3-deoxy-l-galactonate; agarose; red algae; 3; 6-anhydro-l-galactose
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Funding
- Mid-career Researcher Program through the National Research Foundation of Korea [2020R1A2B5B02002631]
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry - Ministry of Agriculture, Food, and Rural Affairs [32136-05-1-SB010]
- Korea University Food Safety Hall
- Korea University Grant
- LG Chem grant
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2-keto-3-deoxy sugar acids, including 2-keto-3-deoxy-l-galactonate (KDGal), can be biologically produced from plant-derived pectin and agarose. In this study, KDGal was enzymatically produced from agarose using the catabolic pathway of 3,6-anhydro-l-galactose (AHG), and purified by size-exclusion chromatography. This research demonstrates the potential of enzymatic production and purification of KDGal from agarose for high-value product synthesis.
2-keto-3-deoxy sugar acids, which have potential as precursors in medicinal compound production, have gained attention in various fields. Among these acids, 2-keto-3-deoxy-l-galactonate (KDGal) has been biologically produced from D-galacturonate originating from plant-derived pectin. KDGal is also found in the catabolic pathway of 3,6-anhydro-l-galactose (AHG), the main component of red-algae-derived agarose. AHG is converted to 3,6-anhydrogalactonate by AHG dehydrogenase and subsequently isomerized to KDGal by 3,6-anhydrogalactonate cycloisomerase. Therefore, we used the above-described pathway to produce KDGal from agarose. Agarose was depolymerized to AHG and to agarotriose (AgaDP3) and agaropentaose (AgaDP5), both of which have significantly higher molecular weights than AHG. When only AHG was converted to KDGal, AgaDP3 and AgaDP5 remained unreacted. Finally, KDGal was effectively purified from the enzymatic products by size-exclusion chromatography based on the differences in molecular weights. These results show that KDGal can be enzymatically produced and purified from agarose for use as a precursor to high-value products.
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