Journal
MARINE DRUGS
Volume 20, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/md20040226
Keywords
macrodiolide; halosmysins; Halosphaeriaceae sp; marine alga; cytotoxicity
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Two new cytotoxic metabolites, named halosmysin B and C, have been discovered from a fungus isolated from a marine alga. The chemical structures and absolute stereostructures of these compounds were determined. Halosmysin B exhibited potent cytotoxicity against leukemia cell lines.
Two new cytotoxic metabolites, halosmysins B and C, have been isolated from the fungus Halosphaeriaceae sp. OUPS-135D-4 separated from the marine alga Sargassum thunbergii. These chemical structures have been elucidated by 1D and 2D NMR, and HRFABMS spectral analyses. The new compounds had the same 14-membered macrodiolide skeleton as halosmysin A, which was isolated from this fungal strain previously. As the unique structural feature, a diketopiperazine derivative and a sugar are conjugated to the 14-membered ring of halosmysins B and C, respectively. The absolute stereostructures of them were elucidated by the chemical derivatization such as a hydrolysis, the comparison with the known compounds (6R,11R,12R,14R)-colletodiol and halosmysin A, and a HPLC analysis of sugar. In addition, their cytotoxicities were assessed using murine P388 leukemia, human HL-60 leukemia, and murine L1210 leukemia cell lines. Halosmysin B was shown to be potent against all of them, with IC50 values ranging from 8.2 +/- 1.8 to 20.5 +/- 3.6 mu M, though these values were slightly higher than those of halosmysin A.
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