Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 160, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2022.113307
Keywords
Ultrasound-assisted enzymatic method; Sea cucumber gonadal polysaccharides; Physicochemical properties; Antioxidant activity
Categories
Funding
- Major Science and Technology Projects of Millions Engineering in Heilongjiang Province [2019ZX07B03]
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This study investigated the physicochemical properties and antioxidant activity of sea cucumber gonadal polysaccharide extracted using enzymatic, ultrasound, and ultrasound-assisted enzymatic methods. The results showed that the polysaccharide extracted by the ultrasound-assisted enzymatic method had higher total sugar and sulfate group content compared to other extraction methods. The antioxidant activity of the ultrasound-assisted enzymatic extracted polysaccharide was also higher in the tested concentration range.
The physicochemical properties and antioxidant activity of sea cucumber gonadal polysaccharide extracted by enzymatic, ultrasound and ultrasound-assisted enzymatic methods were investigated. The results showed that the total sugar and sulphate group content (69.1 g/100 g, 14.7 g/100 g) of SCGP-UAE were higher than those of SCGP-E (58.8 g/100 g, 10.6 g/100 g) and SCGP-U (62.9 g/100 g, 13.3 g/100 g). The zeta potential, molecular weight and particle size of SCGP-UAE were significantly lower than those of SCGP-E and SCGP-U (P < 0.05). Their molar ratios of monosaccharide composition were different. With the concentration of SCGP-E, SCGP-U and SCGP-UAE from 0.25 increased to 4.00 mg/mL, the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot), hydroxyl radical (center dot OH) scavenging ability and reducing power of them were improved. In the concentration range of 0.50-4.00 mg/mL, the antioxidant ability of SCGP-UAE was higher than SCGP-E and SCGP-U. At 4.00 mg/mL, the DPPH center dot, center dot OH scavenging ability and reducing power of SCGP-UAE were 12.8%, 14.6% and 9.42% higher than those of SCGP-U and 38.1%, 29.9% and 29.3% higher than those of SCGP-E, respectively. The strongest anti-oxidant activity of SCGP-UAE was due to the UAE method degraded SCGP chains and exposed more sulphate groups by breaking glycosidic bonds within SCGP.
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