Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 159, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2022.113204
Keywords
Enzyme; Surfactant; Mineral; Kinetics; Fatty acids
Categories
Funding
- National Key Research and Development Program of China [2021YFA0910400]
- China Post-doctoral Science Foundation [2019M662298, 2019M652325]
- Qingdao Postdoctoral Applied Research Project
- Scientific Research Starting Foundation of Qingdao University
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Cetyl trimethyl ammonium bromide (CTAB)-activated lipase-mineral hybrid microflowers (CTAB-Lip-MHFs) showed good substrate affinity and stability for fatty acid production through hydrolysis of olive oil.
Cetyl trimethyl ammonium bromide (CTAB)-activated lipase-mineral hybrid microflowers (CTAB-Lip-MHFs) were fabricated via biomineralization using Cu-3(PO4)(2)center dot 3H(2)O as the mineral for olive oil hydrolysis. FT-IR spectra indicated that immobilized lipase molecules in CTAB-Lip-MHFs has good structural integrity, and XPS spectra revealed that immobilized lipase molecules were present at the surface of CTAB-Lip-MHFs. Kinetic studies suggested that CTAB-Lip-MHFs had good substrate affinity ascribed to interfacial effect caused by CTAB, meanwhile, the open conformation of lipase activated by CTAB contributed to increased reaction rate with substrate. The generated fatty acids via hydrolysis of olive oil by CTAB-Lip-MHFs were 146% of that by free lipase, moreover, CTAB-Lip-MHFs could retain 76.5% and 93.2% of initial activities after storage at 50 degrees C for 10 h and 4 degrees C for 30 days, respectively, indicating the good stability of CTAB-Lip-MHFs. In addition, after 5 recycles, CTAB-Lip-MHFs preserved 72.7% of initial activity, demonstrating the favorable reusability of CTAB-Lip-MHFs for fatty acid production.
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