Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 163, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2022.113532
Keywords
Extracellular polysaccharides; Bacillus thuringiensis; Cytotoxicity; Gut microbiota; Prebiotic
Categories
Funding
- National Key Research and Devel-opment Program of China [2018YFC1604105-5]
- Na-tional Key Research and Development Program of China [2021YFC2101100]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20_1827]
- Program of Introducing Talents of Discipline to Universities [111-2-06]
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The extracellular polysaccharide BPS-1 produced by Bacillus thuringiensis has potential prebiotic properties, as it enhances the abundance of beneficial bacteria and promotes the production of short-chain fatty acids in vitro.
Microbial extracellular polysaccharides possess various beneficial characteristics and are potential prebiotic candidates. The extracellular polysaccharide BPS-1 was isolated from Bacillus thuringiensis (its production being optimized by response surface methodology) and subjected to high-cell-density fermentation. BPS-1 is a heteropolysaccharide (molecular weight 35.6 kDa) composed of rhamnose, arabinose, glucosamine, galactose, glucose, and mannose in respective molar ratios 3.7:1.7:4.5:1:3.1:1.1. Its functional groups and structural features were characterized by FT-IR and NMR. The cytotoxicity of BPS-1 was evaluated against HcoEpiC and LoVo cell lines. BPS-1 was also evaluated for its potential effects on human gut microbiota in an in vitro fermentation model. During 48 h of anaerobic fermentation by healthy human fecal bacteria, BPS-1 significantly enhanced the production of short-chain fatty acids and increased the relative abundance of beneficial bacterial genera. This is the first report of the prebiotic potential of BPS-1 produced by Bacillus thuringiensis and provides a basis for its application in the food industry.
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