Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 163, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2022.113587
Keywords
Paeonol; Aspergillus flavus; Ergosterol biosynthesis; Redox metabolism; Aflatoxin biosynthesis
Categories
Funding
- Innovative Funds Plan of Henan University of Technology [2020ZKCJ17]
- Natural Science Research Projects of Education Department of Henan Province [21A550005]
- Doctor Research Fund of Henan University of Technology [2019BS019]
- Zhengzhou Key Science and Technology Innovation Project [2020CXZX0077]
- National Innovation and Entrepreneurship Training Program for College Students [202110463038]
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This study found that paeonol can inhibit the growth of A. flavus by reducing ergosterol content and perturbing the redox system. It also effectively restrains the production of aflatoxins. RNA-seq analysis revealed that paeonol regulates the expression of key genes involved in ergosterol biosynthesis, redox system, oxylipin synthesis, global regulation, and aflatoxin biosynthesis.
Aspergillus flavus (A. flavus) is one pathogenic fungus that severely endangers human health and food safety. In our previous work, paeonol, a natural active compound from Paeonia. spp can inhibit the growth of A. flavus, and the mode of action is involved with targeting cell walls and cell membranes. In the present work, we have found out that paeonol reduced ergosterol content and perturbed the redox system of A. flavus. Furthermore, paeonol effectively restrained the production of both aflatoxin B1 and aflatoxin B2 on A. flavus inoculated rice. To reveal a whole regulation of paeonol on the transcription level, RNA-seq analysis was conducted. In combination with real-time quantitative PCR, the relative expressions of several key genes participating in ergosterol biosynthesis (erg1, erg4, erg5, erg9, erg11, erg24, and erg25), redox system (cat,mnSOD, and hyr1), oxylipins (ppoA and ppoC), global regulators (veA, velB, and laeA), and aflatoxin biosynthesis (up to 22 genes) were regulated significantly by paeonol treatment. This study not only showed that paeonol could strongly inhibit the growth of A. flavus and the production of its secondary metabolites, but also provided new insights into the antifungal mechanism of paeonol against A. flavus.
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