Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 160, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2022.113333
Keywords
Lipase; Sol-gel biosilicification; Interface immobilization; Hydrolysis of soybean oil
Categories
Funding
- Science and Technology Program of Tianjin, China [20ZYJDJC00080]
- Key Pro-jects of Tianjin Natural Science Foundation, China [19JCZDJC38100]
- National Natural Science Foundation of China [22108206]
- Key R & D projects in Zhongning County, China [2021YBYF0808]
- project of Guangxi Key Research and Development program, China
- project of Key Research and Development of Shandong province, China [2021CXGC010509]
Ask authors/readers for more resources
This study developed a novel silica@lipase hybrid biocatalyst using the sol-gel biosilicification approach, which exhibited superior catalytic activity and storage stability in the hydrolysis of vegetable oils. The hybrid biocatalyst achieved a hydrolysis yield of 98.8% for soybean oil within only 4 hours, and retained 63% of its initial activity after 5 consecutive cycles.
Enzymatic hydrolysis of vegetable oils is crucial for its potential application in foods and drugs. However, the poor activity of free lipase hinders the reaction. Herein, inspired by lipase interface activation, a novel silica@lipase hybrid biocatalyst with a superior catalytic activity for hydrolysis of vegetable oils was developed though the sol-gel biosilicification approach in the oleic acid/water two-phase system. The resultant silica@lipase hybrid biocatalyst exhibited 13.5-fold higher activity than free lipase, and excellent storage stability. After 100 days of storage, the free lipase absolutely loss hydrolytic activity whereas the silica@lipase hybrid biocatalyst still retained 197.4% of its initial activity. In particular, hydrolysis yield of soybean oil by the hybrid biocatalyst reached 98.8% within only 4 h, which is the fastest value recorded in the current literature. After 5 consecutive cycles, hydrolysis yield still retained up to 63%. Therefore, this strategy provides a facile and efficient method for preparing lipase hybrid biocatalysts with high hydrolysis activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available